Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8650085 | Journal of Thermal Biology | 2018 | 32 Pages |
Abstract
We investigated the effects of heat stress on genetically improved farmed tilapia, focusing on metabolic and immune responses. Differences in blood parameters, serum biochemistry, muscle fatty acid composition, and microRNA (miRNA) expression were analyzed in fish under heat stress. Fish were exposed to heat stress at 35â¯Â°C and sampled at 0, 6, 12, 24, and 48â¯h after exposure and compared with a control group maintained at 28â¯Â°C. The results showed that red and white blood cell counts, hemoglobin levels, and hematocrit values tended to increase (Pâ¯<â¯0.05) and reached their maximum levels after 24â¯h, then declined. Acute heat stress enhanced serum glucose, total protein, and total cholesterol levels, and muscle fatty acid components were also altered. Serum alanine aminotransferase (ALT) activity was significantly increased after heat stress for 6 and 12â¯h. Polyunsaturated fatty acids levels were increased after heat stress for 12 and 24â¯h, whereas levels of monounsaturated fatty acids decreased in response to heat stress. Expression of hepatic miR-1 and miR-122 was significantly upregulated, and expression of miR-10c was significantly increased (Pâ¯<â¯0.05) only after heat stress for 48â¯h. Acute heat stress altered metabolism closely related to the immune system and the liver of tilapia. These findings contribute to a theoretical framework for tilapia breeding at high temperatures.
Keywords
Related Topics
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Agricultural and Biological Sciences
Agricultural and Biological Sciences (General)
Authors
Jing-Wen Bao, Jun Qiang, Yi-Fan Tao, Hong-Xia Li, Jie He, Pao Xu, De-Ju Chen,