Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8875145 | Annals of Agricultural Sciences | 2017 | 9 Pages |
Abstract
The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured at concentrations of 0.01-2.56â¯mg/mL of grape and mulberry leaves ethanolic extracts. The MIC values were ranged from 0.08 to 0.16â¯mg/mL against Ps. aeruginosa Ps9, and 0.32â¯mg/mL against each of S. aureus St3, E. coli Ec3, and S. typhi Sa1. Whereas, the MBC values were ranged from 0.32 to 1.28â¯mg/mL of the tested extracts. The effects of the tested extracts were also studied representing the bactericidal effect of the grape extract with a ratio of 2 against all investigated isolates, except S. typhi Sa1. Whereas, the mulberry extract had a bactericidal effect towards S. aureus St3 and E. coli Ec3 with ratio of 2, and a bacteriostatic effect against Ps. aeruginosa Ps9 and S. typhi Sa1 with a ratio â¥4. The investigated bacteria found to have a strong ability to form biofilms with densities ranged from 0.67 to 0.80. Both tested extracts inhibited these biofilms with percentages ranged from 48 to 66% at sub-inhibitory concentrations (SICs) ranged from 0.04 to 0.16â¯mg/mL. In addition, the tested extracts have an excellent cytotoxic activity towards colon cancer cell lines (HCT-16). Five phenolic compounds detected in the tested extracts of grape and mulberry using high performance liquid chromatography (HPLC) after 9.53â¯min of the retention time. The phenolic compounds of both tested extracts were gallic, coumaric, ferulic, chlorogenic and caffeic with concentrations ranged from 1.28 to 6.56â¯Âµg/mL.
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Agricultural and Biological Sciences (General)
Authors
Elshahat M. Ramadan, Khadiga A. Abou-Taleb, Gehan F. Galal, Nermin S. Abdel-Hamid,