Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8875210 | The Egyptian Journal of Aquatic Research | 2018 | 7 Pages |
Abstract
In the present study, juice of water hyacinth (Eichhorina crassipes), either crude or from its successive dilutions (1:1, 1:10, 1:30, 1:50 v/v) supported the in vitro development of Bacillus megaterium, Bacillus subtilis, Azotobacter chroococcum and Rhizobium leguminosarum biovar Phaseoli with doubling time (23.1-63.0â¯min) which was comparable if not shorter, to that calculated using the standard laboratory -synthetic media (nutrient, N-deficient mannitol and yeast extract agar media; 48.0-64.8â¯min). Rhizospheric microorganisms of legume and non-legume plants successfully grew on surface-inoculated agar plates of crude and diluted juices of the macrophyte. Tea bags filled with the dehydrated powders (5 and 10â¯gâ¯lâ1) of water hyacinth supported the in situ recoverability of total rhizobacteria in population densities (3â¯Ãâ¯107 - >108â¯cfu.gâ1), which were found to be comparable, if not excessive, to those enumerated on the recommended culture media. Morpho-physiological identification of some isolates that had developed on the plant juice and tea bag culture media, revealed that they are not akin to those cultured on the chemically-synthetic culture media; they possibly represent a portion of recommended media - unculturables.
Keywords
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Agricultural and Biological Sciences
Agricultural and Biological Sciences (General)
Authors
Rasha H. Ahmed, H. Mona Badawi, Abdallah S. Ali, Mohammed Fayez,