Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8879510 | Industrial Crops and Products | 2018 | 6 Pages |
Abstract
In the present study, a liquid-liquid extraction strategy for sample pretreatment and solvent system selection was established to assist comprehensive separation of major active compositions from natural product by counter-current chromatography. The two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water at the ratio of 10:0:8:2, 5:5:5:5 and 3:5:3:5 (v/v) were used for sample pretreatment, in which the major compositions were almost entirely distributed in the upper phase of two-phase solvent systems. After extraction, the enriched components FI, FV and FVI were obtained. According to this result, the two-phase solvent systems were slightly adjusted to lower polarities and used for HSCCC separation. As a result, 62âmg of compound 6 was isolated from 200âmg FI. 15âmg of compound 4 and 13âmg of compound 5 were isolated from 70âmg FV. 15âmg of compound 3, 51âmg of compound 1 and 16âmg of compound 2 were isolated from 200âmg FVI. The purities of all isolated compounds were over 93%. Their structures were identified as p-hydroxybenzoic acid, kaempferol-3-O-β-d-glucopyranoside, kaempferol 7-O-α-l-rhamnopyranoside, formononetin, 4â²-O-methyl kaempferol, and dibutyl phthalate by 1H NMR and 13C NMR.
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Authors
Jiao Yang, Dongyu Gu, Zhenni Ji, Chen Fang, Fa Xu, Yi Yang,