Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8882209 | South African Journal of Botany | 2018 | 7 Pages |
Abstract
The present work reports investigation on phenolic compounds, antioxidant activity and enzyme inhibitory activities (acetylcholinesterase, butyrylcholinesterase, tyrosinase, α-amylase and α-glucosidase) of different extracts from two Moroccan Asteraceae species; Bubonium imbricatum Cav. and Cladanthus arabicus (L.) Cass. B. imbricatum extracts contained the highest amounts of phenolics and flavonoids, and also exhibited higher antioxidant activity. In this species, the highest total phenolic (1611.13â¯Â±â¯14.23â¯Î¼molGAE/gextract) and flavonoid (376.11â¯Â±â¯8.22â¯Î¼molQE/gextract) contents were observed in aqueous-methanol extract obtained by maceration. Further, UHPLC-MS analysis of C. arabicus and B. imbricatum extracts revealed the presence of several flavonoids (diosmetin, luteolin, apigenin 7-glucoside, and apigenin) and phenolic acids (benzoic, protocatechuic, p-coumaric, gallic, vanillic, caffeic, ferulic and isochlorogenic acids). The antioxidant activity of the extracts was dependent of the extraction process and solvent used. Aqueous-methanol extract of B. imbricatum prepared by maceration showed the highest activity with DPPH, ABTS and FRAP tests (respectively: IC50â¯=â¯8.53â¯Â±â¯0.38â¯Î¼g/ml, 3461.8â¯Â±â¯9.38â¯Î¼molTE/gextract and 3281.6â¯Â±â¯47.43â¯Î¼molAAE/gextract). The results indicated that most of the tested extracts or essential oils exhibited activity towards the tested enzymes. Overall, the results obtained in this work indicated the two Moroccan species studied, particularly B. imbricatum, as valuable sources of natural agents beneficial for human health.
Keywords
TFCBTCIFolin–Ciocalteu reagentF–C reagentbutyrylthiocholineBChEbutyrylcholinesteraseATCIDTNBGAETEACTPCFRAPTCADPPHBHTABTS2,2-diphenyl-1-picrylhydrazyl5,5′-dithiobis(2-nitrobenzoic acid)6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acidUHPLC–MSα-Amylaseα-GlucosidaseAChEAcetylthiocholine iodideUltrasound-assisted extractionAcetylcholinesterasetrichloroacetic acidgallic acid equivalentsUAEAlzheimer's diseaseTrolox EquivalentsTroloxTyrosinaseTrolox equivalent antioxidant capacityPhenolicsMethanolTotal phenolic contentTotal flavonoid contentMeOHbutylated hydroxytolueneCHECholinesterase
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Authors
A. Aghraz, S. Gonçalves, R. RodrÃguez-Solana, L. Ait Dra, V. Di Stefano, G. Dugo, N. Cicero, M. Larhsini, M. Markouk, A. Romano,