Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8883682 | Aquatic Toxicology | 2018 | 43 Pages |
Abstract
Despite a wide number of toxicological studies that describe benzo[a]pyrene (BaP) effects, the metabolic mechanisms that underlie these effects in fish are largely unknown. Of great concern is the presence of BaP in aquatic systems, especially those in close proximity to human activity leading to consumption of potentially contaminated foods. BaP is a known carcinogen and it has been reported to have adverse effects on the survival, development and reproduction of fish. The purpose of this study was to investigate if a low dose of BaP can alter genes and key metabolic pathways in the liver and testis in male adult tilapia, and whether these could be associated with biological endpoints disruption. We used both high-throughput RNA-Sequencing to assess whole genome gene expression following repeated intraperitoneal injections of 3â¯mg/kg of BaP (every 6 days for 26 days) and morphometric endpoints as indicators of general health. Condition factor (K) along with hepatosomatic and gonadosomatic indices (morphometric parameters) were significantly lower in BaP-treated fish than in controls. BaP exposure induced important changes in the gene expression pattern in liver and testis as revealed by both Pathway and Gene Ontology (GO) analyses. Alterations that were shared by both tissues included arachidonic acid metabolism, androgen receptor to prostate-specific antigen signaling, and insulin-associated effects on lipogenesis. The most salient liver-specific effects included: biological processes involved in detoxification, IL6-associated insulin resistance, mTOR hyperactivation, mitotic cytokinesis, spindle pole and microtubule binding. BaP effects that were confined to the testis included: immune system functions, inflammatory response, estrogen and androgen metabolic pathways. Taken together, gene expression and morphometric end point data indicate that the reproductive success of adult male tilapia could be compromised as a result of BaP exposure. These results constitute new insights on the mechanism of action of low dose BaP in a non-model organism (tilapia).
Keywords
CD38Shp1IAPTGFNGFRAPAF1CytCDIABLOTIRAPATM/ATRFLIPNIKTNFRJanus kinase 1STCMAP3KCaMKKSMADNF-kBinterleukin 1 receptor type 1PI3KIL1IL1Rp53CASP9RNA-seqPDPK1TLRAKT serine/threonine kinaseIL1RL1pKaNGFTGFBRTNFRyRcAMPadenylate cyclaseAktStanniocalcin 1interleukin 1Benzo(a)pyrenetransforming growth factortransforming growth factor beta receptor 1toll like receptortumor protein p53TilapiaBcl-2 familycytochrome cnerve growth factorApoptotic Peptidase Activating Factor 1tumor necrosis factorphosphatase and tensin homologTranscriptomicsNogoprotein kinase ABIDPiPJAKPtenCaspase 9Ryanodine receptorCytokine receptornerve growth factor receptor
Related Topics
Life Sciences
Agricultural and Biological Sciences
Aquatic Science
Authors
Reyna Cristina Colli-Dula, Xiefan Fang, David Moraga-Amador, Nacira Albornoz-Abud, Roberto Zamora-Bustillos, Ana Conesa, Omar Zapata-Perez, Diego Moreno, Emanuel Hernandez-Nuñez,