Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8883712 | Aquatic Toxicology | 2018 | 37 Pages |
Abstract
Bisphenol A has attracted worldwide attention due to its harmful effects on humans, animals and plants. In this study, the toxicological effects of BPA on Cylindrospermopsis raciborskii were assessed based on chlorophyll a ï¬uorescence and transcriptome analyses. The results showed that the growth of C. raciborskii was significantly inhibited when BPA exceeded 0.1â¯mgâ¯Lâ1. A marked rise of phase J was observed at a concentration greater than 0.1â¯mgâ¯Lâ1, while a K phase appeared at 20â¯mgâ¯Lâ1. The chlorophyll a ï¬uorescence parameters of RC/CS0, F0, ÏP0, ÏE0, and Ï0, underwent a significant decline under all treatments of BPA, whereas a significant increase in both VJ and M0 occurred under all concentrations of BPA. Additionally, ABS/RC and DIo/RC markedly increased at 10â¯mgâ¯Lâ1 and 20â¯mgâ¯Lâ1. The transcriptome analysis revealed that the genes of photosynthesis, including psbA, psbB, psbC, psbD, apcA, apcB, cpcA, and cpcB, as well as those of chlorophyll and carotenoid biosynthesis, namely hemN, acsF, chlL, chlN, chlP, crtB, pds, were all down-regulated. Moreover, BPA also inhibited the oxidative phosphorylation, glycolysis/gluconeogenesis, citrate cycle (TCA cycle), and fatty acid metabolism in C. raciborskii. Taken together, these results suggest BPA can negatively affect the expression of multiple genes and the vital energy metabolism process to arrest the growth and photosynthesis of C. raciborskii.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Aquatic Science
Authors
Rong Xiang, Junqiong Shi, Hongbo Zhang, Congcong Dong, Li Liu, JunKe Fu, Xinyu He, Yanjun Yan, Zhongxing Wu,