Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8887024 | Environmental and Experimental Botany | 2018 | 12 Pages |
Abstract
Onion (Allium cepa) seeds gradually lose vigor during storage, even under optimal conditions. Some of the loss in vigor is reversible and can be recovered by priming. Electrostatic field priming can instantly increase seed vigor and accelerate germination, though the effects are short-lived. Hydro-priming is most widely used as it does not use chemicals and is a clean process, but requires a long priming time and precise seed moisture control. Here, we investigated the potential of combined hydro-electrostatic hybrid priming on the recovery of vigor in onion seeds. Hydro-electrostatic hybrid priming lead to improved seed vigor recovery. Circular dichroism spectroscopy and electron paramagnetic resonance revealed exposure to an electrostatic field enhanced SOD activity in seeds by changing the secondary structure of the enzyme and led to more efficient free radical scavenging; scanning and transmission microscopy revealed these effects promoted more complete and rapid healing of embryo cell organelles and plasma membranes during the incubation stage of hydro-priming. Hydro-electrostatic hybrid priming has the advantages of greater recovery of potential vigor and a shorter processing time than single hydro-priming, leads to longer lasting effects than single electro-priming, and does not require any chemicals.
Keywords
MDAPBSPCAPrimingsodium dodecyl sulfate-polyacrylamide gel electrophoresisSDS-PAGETemSeedPrincipal component analysisHybridEPRElectron paramagnetic resonancecircular dichroismSODSuperoxide dismutasemalondialdehydePhosphate-buffered salineSEMElectric fieldScanning electron microscopyTransmission electron microscopyHydro
Related Topics
Life Sciences
Agricultural and Biological Sciences
Ecology, Evolution, Behavior and Systematics
Authors
Yinglei Zhao, Minghe Hu, Zhao Gao, Xiaoxue Chen, Danfeng Huang,