Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8892218 | Horticultural Plant Journal | 2017 | 11 Pages |
Abstract
In this study, an efficient plant regeneration protocol in vitro and transformation by Agrobacterium-mediated method of Camellia sinensis was achieved, which would lay the foundation for genetic improvement of tea plant by genetic engineering technology. The cotyledon callus of C. sinensis were used as the receptors for transformation by Agrobacterium tumefaciens EHA105 containing PS1aG-3. Some factors which affected the result of Agrobacterium-mediated transformation of C. sinensis were studied on the basis of GUS transient expression system. The optimum system of Agrobacterium-mediated transformation was that the cotyledon callus were pre-cultured for 3âd, and then infected by EHA105 for 15âmin followed by 3âd co-culture in the dark on the YEB medium containing 150âµmolâ
Lâ1 acetosyringone (AS). The transient expression rate of GUS gene was 62.6%. After being delayed selective culture for 3âd, infected callus were transferred into the differentiation medium and the root induction medium both of which were supplemented with 100âmgâ
Lâ1 spectinomycin, and then resistant seedlings of C. sinensis were obtained. The conversion rate was 3.6%.
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Authors
LV Qianru, CHEN Changsong, XU Yijuan, HU Shunkai, WANG Le, SUN Kang, CHEN Xuan, LI Xinghui,