Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8892235 | Horticultural Plant Journal | 2017 | 4 Pages |
Abstract
Bottle gourd is an important cucurbit crop worldwide. To provide more available molecular markers for this crop, a bioinformatic approach was employed to develop insertion-deletions (InDels) markers in bottle gourd based on restriction site-associated DNA sequencing (RAD-Seq) data. A total of 892 Indels were predicted, with the length varying from 1âbp to 167âbp. Single-nucleotide InDels were the predominant types of InDels. To validate these InDels, PCR primers were designed from 162 loci where InDels longer than 2âbp were predicated. A total of 112 InDels were found to be polymorphic among 9 bottle gourd accessions under investigation. The rate of prediction accuracy was thus at a high level of 72.7%. DNA fingerprinting for 4 cultivars were performed using 8 selected Indels markers, demonstrating the usefulness of these markers.
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Authors
WU Xinyi, XU Pei, WU Xiaohua, WANG Baogen, LU Zhongfu, LI Guojing,