Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8892261 | Horticultural Plant Journal | 2016 | 10 Pages |
Abstract
To study the functions of the early auxin-responsive genes CsGH3.1 and CsGH3.6 in citrus resistance against canker disease, we cloned CsGH3.1 and CsGH3.6 in 'Newhall' Navel Orange (Citrus sinensis Osbeck). They are 1â797âbp and 1â887âbp and encode 598 and 629 amino acids, respectively. In vitro mature leaves from susceptible 'Newhall' and resistant Calamondin (C. madurensis) were inoculated by a Xanthomonas axonopodis pv. citri (Xac) bacterial suspension, and expression of CsGH3.1 and CsGH3.6 in the two varieties were analyzed using quantitative real-time PCR (qRT-PCR). 'Newhall' leaves were treated with different hormones for 3âdays, inoculated by Xac bacterial suspension, and then the symptoms in these leaves were investigated. We used qRT-PCR to analyze the effect of different hormones on CsGH3.1 and CsGH3.6 expression in 'Newhall' leaves. The expression levels of both CsGH3.1 and CsGH3.6 were significantly induced by Xac in 'Newhall' leaves, compared with levels in Calamondin leaves. 1-naphthy acetic acid (NAA) increased the hypertrophy of infection sites in 'Newhall' leaves, while naphthyl-phthalamic acid (NPA) had no visible effect on lesion development. NAA hormone greatly improved expression of CsGH3.1 in 'Newhall', but not CsGH3.6. These results indicate that the auxin primary-response gene CsGH3.1 plays an important role in citrus susceptibility to Xac.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Forestry
Authors
CHEN Min, HE Yongrui, XU Lanzhen, PENG Aihong, LEI Tiangang, YAO Lixiao, LI Qiang, ZHOU Pengfei, BAI Xiaojing, DUAN Minjie, JIANG Xueyou, JIA Ruirui, ZOU Xiuping, CHEN Shanchun,