Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8892262 | Horticultural Plant Journal | 2016 | 6 Pages |
Abstract
Six plant growth regulators frequently used in fruit were detected using UPLC-MS/MS including chlormequat chloride, GA3, forchlorfenuron, paclobutrazol, thidiazuron and 2,4-D. A modified QuEChERS method was used in pretreatment: each compound was extracted with acetonitrile containing 1% acetic acid and cleaned with a mixture of ostade-cylsilane (ODS) and MgSO4. The detection was performed using electrospray ionization (ESI) and multiple reaction monitoring mode (MRM) and quantified using the external standard method. Results indicated that the calibration curves of six plant growth regulators were linear within the range of 1-100âµgâ
kg-1 with correlation coefficients over 0.999. The recovery at four spiked concentrations, 1, 20, 50, and 100âµgâ
kg-1, ranged from 60.77% to 119.43% and relative standard deviation (RSD) were between 0.18% and 32.94%. The limits of detection (LOD) and limits of quantitation (LOQ) were 0.01-0.14âµgâ
kg-1 and 0.02-0.46âµgâ
kg-1, respectively, for apples, 0.01-0.18âµgâ
kg-1 and 0.03-0.59âµgâ
kg-1 for grapes, 0.01-0.08âµgâ
kg-1 and 0.04-0.28âµgâ
kg-1 for kiwis, 0.003-0.110âµgâ
kg-1 and 0.01-0.38âµgâ
kg-1 for peaches, and 0.01-0.08âµgâ
kg-1 and 0.03-0.25âµgâ
kg-1 for oranges.
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Authors
Yan Zhen, Nie Jiyun, Xu Guofeng, Li Haifei, Li Jing, Li Zhixia, Wu Yonglong, Kuang Lixue,