Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8965649 | Ticks and Tick-borne Diseases | 2018 | 4 Pages |
Abstract
This study investigated the target site mutations in the partial sequence of voltage-sensitive sodium channel (VSSC) domain II of synthetic pyrethroid (SP)-resistant Rhipicephalus appendiculatus. Genomic DNA was extracted from seven tick populations (two susceptible and five resistant) collected from central, eastern and southwestern Uganda. The PCR amplicons of the VSSC domain II were cloned and sequenced to determine novel single nucleotide polymorphisms (SNP). A non-synonymous mutation C78âA corresponding to C190âA was found in all the five SP-resistant ticks. The C78âA mutation led to amino acid substitution from leucine to isoleucine (L21I) which was previously reported to confer knockdown (kdr) mutation in R. (Boophilus) microplus. The genetic confirmation of SP-resistant R. appendiculatus in central and southwestern Uganda calls for an urgent strategy for controlling the ticks.
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Authors
Patrick Vudriko, Rika Umemiya-Shirafuji, James Okwee-Acai, Dickson Stuart Tayebwa, Joseph Byaruhanga, John Son Bbira, Kozo Fujisaki, Xuenan Xuan, Hiroshi Suzuki,