Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8967111 | Domestic Animal Endocrinology | 2005 | 8 Pages |
Abstract
Goat preantral follicles were cultured to investigate the effects of insulin-like growth factor-I (IGF-I), epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) on the in vitro growth and viability of oocytes. Preantral follicles were isolated mechanically and enzymatically (using collagenase and DNase) from prepuberal goat ovaries. The working medium was composed of Defined Eagle's Minimum Essential Medium (DMEM) supplemented with HEPES (20Â mM), 10% fetal calf serum (FCS), hypoxanthine (2Â mM), dibutyryl cyclic adenosine 3â²,5â²-monophosphate (dbcAMP) (2Â mM), penicillin (75Â ng/ml) and streptomycin (50Â ng/ml). The culture medium consisted of the working medium with follicle stimulating hormone (FSH) (100Â ng/ml) and hydrocortisone (40Â ng/ml) added. In the experiment, goat preantral follicles were cultured for 9 days in the culture medium and in the culture medium supplemented with either IGF-I (100Â ng/ml), EGF (50Â ng/ml), bFGF (50Â ng/ml) or IGF-I (100Â ng/ml)Â +Â EGF (50Â ng/ml). The results indicated that IGF-I (100Â ng/ml) effectively maintained the survival of oocytes and promoted their growth; EGF (50Â ng/ml) enhanced the survival rate of oocytes but had a negative effect on oocyte growth; bFGF (50Â ng/ml) stimulated oocyte survival but had no obvious effect on their growth while IGF-I (100Â ng/ml) and EGF (50Â ng/ml) in combination had a greater effect on both survival and growth rate of oocytes than IGF-I or EGF alone. The supplementation of IGF-1 and EGF to the culture medium is recommended in the culture of goat preantral follicles.
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Authors
Huanmin Zhou, Yong Zhang,