Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8974898 | Aquaculture | 2005 | 8 Pages |
Abstract
The yellowtails Seriola quinqueradiata and Seriola lalandi are the most important species in marine fishery resources and aquaculture in Japan. A genetic linkage map is needed to improve efficiency of breeding by marker-assisted selection (MAS) and for the identification of commercially important genes. Therefore, we have constructed a genetic linkage map for the yellowtails using microsatellite markers. Microsatellites were isolated from a genomic DNA library of S. quinqueradiata. Segregation of 217 microsatellites was studied in 90 progeny from a cross between S. quinqueradiata and S. lalandi. These were used to construct separate linkage maps of a female (S. quinqueradiata) and a male (S. lalandi). Twenty-five linkage groups were distinguished in the female (S. quinqueradiata) map, which spanned for 473.3 centiMorgans (cM) with an average intermarker distance of 2.7 cM (total length estimated: 901.7 cM), and 21 linkage groups were distinguished in the male (S. lalandi) map, which spanned 584.3 cM with an average intermarker distance of 4.8 cM (total length estimated: 1715.3 cM). The microsatellite loci and genetic linkage maps will increase the efficiency of selective breeding programs for yellowtails.
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Authors
Eriko Ohara, Takuya Nishimura, Yoshitomo Nagakura, Takashi Sakamoto, Keiichi Mushiake, Nobuaki Okamoto,