Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8980686 | Journal of Comparative Pathology | 2005 | 4 Pages |
Abstract
Bone-marrow samples were collected from 48 CAEV-seropositive, symptomless goats (30 kids, 18 adults). The samples were formalin-fixed and processed for histological examination. In addition, all samples were examined immunohistochemically with a monoclonal antibody (1A7) against the p27 capsid protein of maedi-visna virus, an antibody which cross-reacts with the Ca-p27 of CAEV. Samples from 16 goats (10/30 kids, 6/18 adults) showed positive immunolabelling of bone-marrow stromal cells (fibrocytes, endothelial cells and adipocytes) and of scattered macrophages, whereas haematopoietic cells were negative. The detection of viral Ca-p27 protein in bone-marrow fibrocytes was consistent with previous in-vitro studies which indicated that such cells are semi-permissive for CAEV infection. It is speculated that bone-marrow stromal cells represent a viral reservoir in symptomless animals.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Animal Science and Zoology
Authors
P. Grossi, C. Giudice, I. Bertoletti, G. Cioccarelli, E. Brocchi, G. Cammarata, D. Gelmetti,