Quantification of Immune Cell Populations in the Lamina Propria of Equine Jejunal Biopsy Specimens

The histological diagnosis of inflammatory bowel disease (IBD) in horses and other species is subjective, and pathological assessments vary considerably as a result. One important criterion is increased infiltration of the lamina propria by eosinophils, plasma cells, lymphocytes or macrophages, but this is difficult to assess without a knowledge of the normal immune cell populations and potential for individual variation. Retrospective jejunal specimens were analysed from 14 horses aged 13-15 years which had not shown clinical or post-mortem signs of gastrointestinal disease. Populations of plasma cells, T lymphocytes (CD3+), B lymphocytes (CD79a+ cytoplasmic membranes), eosinophils, macrophages and neutrophils were counted in 9000-μm2 areas of the villous lamina propria and intercryptal lamina propria for each horse. There were significantly higher counts of plasma cells, B lymphocytes and eosinophils in the intercryptal than in the villous region, which accords with previous findings in dogs. This information will be used as control data for future quantitative morphometrical analysis of immune cells in small intestinal specimens from horses in which IBD has been diagnosed.