Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8987730 | Veterinary Immunology and Immunopathology | 2005 | 10 Pages |
Abstract
The authors demonstrate for the first time that canine neutrophils derived from Linâ cells are able to produce oxyradicals, express a specific neutrophil surface antigen, and contain gelatinase granules. These characteristics enable them to migrate through basement membranes to act as a first line defence mechanism. The fact that these cells are able to differentiate into functional mature cells, and give rise to long-term culture-initiating cells (LTC-IC) after 35 days of culture, allows the authors to assure that the isolated canine enriched haematopoietic cell population exhibit functional characteristics, associated with primitive haematopoietic cells.
Keywords
FACSColony forming unit-granulocyte-macrophagehepoLTBMCLin−LTC-IChGM-CSFLineage negativeCFU-GMburst forming unit-erythroidBFU-EDHRMWMMMPHuman erythropoietinIn vitro differentiationfluorescent-activated cell sortingdihydrorhodamine 123Rh-123Rhodamine 123phycoerythrinLong-term bone marrow cultureMetalloproteinasemolecular weight markers
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Authors
Leticia G. León, Luciana K. Ostronoff, MarÃa Luisa FermÃn, Cristina FragÃo, Elisabeth Kremmer, Hans-Jochem Kolb, Concepción Tejero,