Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8987931 | Veterinary Immunology and Immunopathology | 2005 | 11 Pages |
Abstract
Previous studies in our laboratory suggested that there was positive selection of B cells during early development in the appendix of normal and VH mutant (ali/ali) rabbits. Preferential expansion and survival of B lymphocytes was affected by the Ig VH frameworks 1 and 3 sequences expressed on the cell surface. We demonstrated a specific interaction between rabbit CD5 and the V region of rabbit heavy chains and suggested that CD5 is a potential selecting ligand for B-cell surface immunoglobulin framework region sequences. To further investigate the role of CD5 in rabbit B-cell selection and survival we prepared recombinant constructs and obtained stable expression of the three scavenger receptor cysteine-rich (SRCR) extracellular domains of rabbit CD5. Here we describe the production and purification of this expressed recombinant CD5 protein, polyclonal antibody obtained by immunization of a goat and initial production and characterization of specific mAbs against peptides selected from each sequenced SRCR domain.
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Authors
Richard Pospisil, Harold Obiakor, Barbara A. Newman, Cornelius Alexander, Rose G. Mage,