Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8991011 | Veterinary Parasitology | 2005 | 4 Pages |
Abstract
A non-isotopic single-strand conformation polymorphism ('cold' SSCP) technique has been assessed for the analysis of sequence variability in the expansion segment 5 (ES5) of domain IV and the D3 domain of nuclear ribosomal DNA within and/or among isolates and individual muscle (first-stage) larvae representing all currently recognized species/genotypes of Trichinella. Data are consistent with the ability of cold SSCP to identify intra-specific as well as inter-specific variability among Trichinella genotypes. The cold SSCP approach should be applicable to a range of other genetic markers for comparative studies of Trichinella populations globally.
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Authors
Robin B. Gasser, Min Hu, Youssef Abs EL-Osta, Dante S. Zarlenga, Edoardo Pozio,