Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8991012 | Veterinary Parasitology | 2005 | 4 Pages |
Abstract
A cDNA library from Trichinella spiralis adults 3 days post-infection was screened with a cDNA probe, designated T54, derived from a newborn larvae subtracted cDNA library. Sequence analysis showed that the positive clone contained a cDNA insert of 1464Â bp in length with a single open reading frame of 1290Â bp, which encoded a protein of 429 amino acids with a putative molecular mass of 49.9Â kDa. Database analysis predicted the deduced protein had a leucine zipper motif and an FYVE zinc finger domain. The recombinant fusion protein was expressed and rabbit anti-recombinant protein sera reacted with a single peptide migrating at approximately 55Â kDa in crude worm extract from muscle larvae, adults and newborn larvae stages.
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Authors
B.Q. Fu, M.Y. Liu, C.M.O. Kapel, X.P. Meng, Q. Lu, X.P. Wu, Q.J. Chen, P. Boireau,