Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8991013 | Veterinary Parasitology | 2005 | 5 Pages |
Abstract
A 5-day-old adult stage-specific cDNA fragment from Trichinella spiralis was identified by suppression subtractive hybridization and was used as a probe to screen the cDNA library. The cDNA sequence coding for a putative T. spiralis cuticle collagen was isolated. The cDNA encoded an open reading frame of 343 amino acid residues with molecular weight of 35.1Â kDa. The deduced protein contained an N-terminal signal peptide, a nematode cuticle collagen N-terminal domain and a collagen triple helix repeat domain. Searches in GenBank using BLASTP showed up to 47% identity to cuticle collagens from other nematodes. Southern blot analysis of genomic DNA indicated this gene was present as a single copy in T. spiralis genome.
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Authors
B.Q. Fu, M.Y. Liu, C.M.O. Kapel, X.P. Meng, Q. Lu, X.P. Wu, Q.J. Chen, P. Boireau,