Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9144592 | Physiological and Molecular Plant Pathology | 2005 | 7 Pages |
Abstract
Agrobacterium vitis causes crown gall disease on grapevines. It also induces a specific necrosis on grape roots and a hypersensitive response (HR) on tobacco that are regulated by a complex quorum-sensing regulatory system. Strain F2/5 produces at least six N-acyl-homoserine lactones (AHLs) that function as signal molecules in quorum-sensing. The AHLs differ in acyl side chain length (8-16 carbons) as determined by gas chromatography/mass spectrometry and electrospray ionization tandem mass spectrometry. Mutant derivatives of F2/5 differ in ability to cause necrosis and the HR and show variable AHL profiles as determined by a thin-layer chromatography/biosensor assay. All wildtype A. vitis strains revealed the presence of long-chain AHLs regardless of tumorigenicity or ability to cause the HR. Whereas genes encoding long-chain AHLs are predicted to reside on the F2/5 chromosome, the determinants for short-chain AHLs were shown to be located on conjugal plasmids.
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Authors
Yaxin Li, Matthew R. Gronquist, Guixia Hao, Michele R. Holden, Anatol Eberhard, Russell A. Scott, Michael A. Savka, Erno Szegedi, Sandor Sule, Thomas J. Burr,