Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9474529 | Journal of Cereal Science | 2005 | 15 Pages |
Abstract
With the combination of these newly emerging technologies, the stage is now set for cereal chemistry to capitalise on advances being made widely in protein chemistry, to apply these new methods, and thereby bridge the traditional gap between DNA and proteins, between the genome and proteome. In doing so, we stand to learn more about the inheritance of grain-quality attributes, and also, possibly more importantly, to discover more about the effect of growth and storage conditions on grain quality, and their effects on processing. In this review, the main aspects of proteomics are discussed, as well as the current and future applications of proteomic technologies to cereal grain science.
Keywords
PSDPuroindolinesPDI2-DEICATPMFTOFIEFPVDFCIDESIDPAMudPITimmobilised pH gradientIPGMS/MSCollision-induced dissociationESTscxtwo-dimensional gel electrophoresisSDS-PAGESodium dodecyl sulfate polyacrylamide gel electrophoresisstrong cation exchangeExpressed Sequence TagBarleypolyvinylidene difluoridedays post-anthesisTime-of-Flight Cultivar identificationMass spectrometryTandem mass spectrometryPost-source decayMatrix-assisted laser desorption/ionisationMALDIIsoelectric pointprotein disulfide isomeraseHeat-shock proteinsliquid chromatographyGrain qualityWheatelectrospray ionisation
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Authors
D.J. Skylas, D. Van Dyk, C.W. Wrigley,