Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9478226 | Aquatic Toxicology | 2005 | 13 Pages |
Abstract
Six strains of marine cyanobacteria, of which five benthic, were isolated from an area of the Portuguese coast with no known apparent toxic microbial bloom. Five strains were lethal for mice. Four of them produced lethargy and four lead to bleeding. One of the toxic strains was from a genus (Aphanothece) not previously associated with toxin production. Extracts from four isolates induced SH-SY5Y-neuroblastoma cell apoptosis without affecting the viability of hepatocytes, NRK kidney cells, or fibroblasts. Aqueous extract from four isolates inhibited thrombin-induced blood platelet activation, with decreased P-selectin expression, platelet aggregation and shedding of platelet-derived micro-vesicles. Curiously, platelets treated with organic extracts from two of the cyanobacterial strains formed platelet micro-vesicles, expressed P-selectin on the surface and showed a distinct phosphotyrosine protein pattern, but failed to aggregate. We conclude that low-abundance marine cyanobacteria growing at low rates may be an important source for novel toxins that may be useful to dissect mammalian signalling pathways of apoptosis and platelet function.
Keywords
FITCACDPLCECLTRAPHRPGFPPMPPI3Kacid citrate dextrosephosphatidic acidenhanced chemiluminescenceToxic cyanobacteriaBenthic cyanobacteriaphycoerythrinPhosphoinositide-specific phospholipase Cphosphoinositide 3-kinasefluorescein isothiocyanateNeuroblastomahorse radish peroxidaseProtein phosphataseBlood plateletsplatelet-derived microparticlesgel-filtered plateletsthrombin receptor agonist peptide
Related Topics
Life Sciences
Agricultural and Biological Sciences
Aquatic Science
Authors
Frode Selheim, Lars Herfindal, Rosario Martins, Vitor Vasconcelos, Stein Ove Døskeland,