Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9488766 | Scientia Horticulturae | 2005 | 6 Pages |
Abstract
This study describes a protocol for rapid and large-scale in vitro propagation of the valuable medicinal herb Vitis thunbergii Sieb. et Zucc.. Culture conditions influencing shoot proliferation and rooting of the two clones (three- and five-lobed) were examined. Three medium formulations, Murashige-Skoog (MS), Woody Plant Medium (WPM) and Nitsch and Nitsch (NN) medium, were tested for growth of shoot tip culture, and WPM was found to have a superior proliferation rate. The chlorophyll content of leaves was highest in those cultured on NN, followed by those on MS and WPM medium. WPM medium supplemented with 0.5 mg lâ1 6-benzyladenine (BA) displayed the highest proliferation rate (15-19 nodes or 3-4 shoots per explant). The rooting was optimized using MS medium supplemented with 0.5 mg lâ1 napthaleneacetic (NAA) with eight roots, 3 cm long after 1 month of culture. High frequency callus formation was observed in the basal end of explants cultured on NAA-containing medium. Following acclimatization, rooting plantlets were transferred to the plastic house with a 95% survival rate.
Keywords
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Agricultural and Biological Sciences
Horticulture
Authors
Mei-Chun Lu,