Ovum pick up and in vitro embryo production in cows superstimulated with an individually adapted superstimulation protocol

The mean number of punctured follicles per session was 11.9 ± 7.7 (mean ± S.D.), with 16% of follicles exceeding 11 mm. These follicles yielded a mean of 5.6 ± 4.1 cumulus oocyte complexes (COC), 32% of which had ≥3 layers of cumulus cells (quality 1 and 2). The recovery rate was 47%. Finally, all COC were subjected to IVM-IVF-IVC, which resulted in a mean of 2.0 ± 2.3 blastocysts on day 7 postinsemination. The subtle changes in pFSH dose influenced the sizes but not the numbers of follicles, the latter parameter was influenced by the individual donor and the OPU session.