Gene synthesis by integrated polymerase chain assembly and PCR amplification using a high-speed thermocycler Fulltext Access 6 Pages 2009
Selection of high affine peptide ligands for detection of Clostridium Tyrobutyricum spores Fulltext Access 6 Pages 2009
Utilizing RNA/DNA hybridization to directly quantify mRNA levels in microbial fermentation samples Fulltext Access 6 Pages 2009
Rapid monitoring of cell size, vitality and lipid droplet development in the oleaginous yeast Waltomyces lipofer Fulltext Access 6 Pages 2009
Novel precipitated fluorescent substrates for the screening of cellulolytic microorganisms Fulltext Access 6 Pages 2009
Multiplex quantification of 16S rDNA of predominant bacteria group within human fecal samples by polymerase chain reaction - ligase detection reaction (PCR-LDR) Fulltext Access 6 Pages 2009
Sterilization of Bacillus pumilus spores using supercritical fluid carbon dioxide containing various modifier solutions Fulltext Access 6 Pages 2009
Detection of Ralstonia solanacearum in natural substrates using phage amplification integrated with real-time PCR assay Fulltext Access 6 Pages 2009
Development of serological proteome analysis of mastitis by Staphylococcus aureus in ewes Fulltext Access 6 Pages 2009
Selective adsorption of hydrocarbon-oxidizing Rhodococcus cells in a column with hydrophobized poly(acrylamide) cryogel Fulltext Access 6 Pages 2009
The mycolyltransferase 85A, a putative drug target of Mycobacterium tuberculosis: Development of a novel assay and quantification of glycolipid-status of the mycobacterial cell wall Fulltext Access 6 Pages 2009
Improvement of a selective media for the isolation of B. anthracis from soils Fulltext Access 6 Pages 2009
Use of resazurin to detect mefloquine as an efflux-pump inhibitor in Pseudomonas aeruginosa and Escherichia coli Fulltext Access 6 Pages 2009
A duplex vibriocidal assay to simultaneously measure bactericidal antibody titers against Vibrio cholerae O1 Inaba and Ogawa serotypes Fulltext Access 6 Pages 2009
Metagenomic study of the oral microbiota by Illumina high-throughput sequencing Fulltext Access 6 Pages 2009
Quantitative cell wall protein profiling of invasive and non-invasive Saccharomyces cerevisiae strains Fulltext Access 6 Pages 2009
Hymenobacter tibetensis sp. nov., a UV-resistant bacterium isolated from Qinghai–Tibet plateau Fulltext Access 6 Pages 2009
Stenotrophomonas chelatiphaga sp. nov., a new aerobic EDTA-degrading bacterium Fulltext Access 6 Pages 2009
Ancylobacter dichloromethanicus sp. nov. – a new aerobic facultatively methylotrophic bacterium utilizing dichloromethane Fulltext Access 6 Pages 2009
Polyphasic taxonomic characterization of Lactobacillus rossiae isolates from Belgian and Italian sourdoughs reveals intraspecific heterogeneity Fulltext Access 6 Pages 2009
Description of Dietzia lutea sp. nov., isolated from a desert soil in Egypt Fulltext Access 6 Pages 2009
Mammal aging: Active and passive mechanisms and their medical implications Fulltext Access 6 Pages 2009
A novel approach for predicting the evolutionary time of acquisition of foreign genes by bacteria: Application of codon usage analyses Fulltext Access 6 Pages 2009
Natural human infections with Vaccinia virus during bovine vaccinia outbreaks Fulltext Access 6 Pages 2009
Hypoxanthine-guanine phosphoribosyltransferase from Mycobacteriumtuberculosis H37Rv: Cloning, expression, and biochemical characterization Fulltext Access 6 Pages 2009
High-yield expression and purification of a monotopic membrane glycosyltransferase Fulltext Access 6 Pages 2009
Clinical and genetic analysis of Human Bocavirus in children with lower respiratory tract infection in Taiwan Fulltext Access 6 Pages 2009
Development and validation of real-time one-step reverse transcription-PCR for the detection and typing of dengue viruses Fulltext Access 6 Pages 2009
Detection of mucosal and cutaneous human papillomaviruses in oesophagitis, squamous cell carcinoma and adenocarcinoma of the oesophagus Fulltext Access 6 Pages 2009
Do rhinoviruses reduce the probability of viral co-detection during acute respiratory tract infections? Fulltext Access 6 Pages 2009
Direct identification of human enterovirus serotypes in cerebrospinal fluid by amplification and sequencing of the VP1 region Fulltext Access 6 Pages 2009
Cytomegalovirus-specific, high-avidity IgG with neutralizing activity in maternal circulation enriched in the fetal bloodstream Fulltext Access 6 Pages 2009
Direct genotyping of cytomegalovirus envelope glycoproteins from toddler's saliva samples Fulltext Access 6 Pages 2009
Evaluation of DNA extraction methods for dried blood spots in the diagnosis of congenital cytomegalovirus infection Fulltext Access 6 Pages 2009
Histological findings in foetuses congenitally infected by cytomegalovirus Fulltext Access 6 Pages 2009
Quantitative multiplex PCR assay for the detection of the seven clinically most relevant high-risk HPV types Fulltext Access 6 Pages 2009
An outbreak of hand, foot, and mouth disease associated with subgenotype C4 of human enterovirus 71 in Shandong, China Fulltext Access 6 Pages 2009
Phylogeny of G9 rotavirus genotype: A possible explanation of its origin and evolution Fulltext Access 6 Pages 2009
Placental endothelial cells can be productively infected by Parvovirus B19 Fulltext Access 6 Pages 2009
Adaptive immune responses against parvovirus B19 in patients with myocardial disease Fulltext Access 6 Pages 2009
Comparison of the performance of the LIAISON VZV-IgG and VIDAS automated enzyme linked fluorescent immunoassays with reference to a VZV-IgG time-resolved fluorescence immunoassay and implications of choice of cut-off for LIAISON assay Fulltext Access 6 Pages 2009
Identification of pathogenic bacteria in blood cultures: Comparison between conventional and PCR methods Fulltext Access 6 Pages 2009
Isolation, characterization, and utilization of a temperature-sensitive allele of a Pseudomonas replicon Fulltext Access 6 Pages 2009
An optimized protocol for the production of interdelta markers in Saccharomyces cerevisiae by using capillary electrophoresis Fulltext Access 6 Pages 2009
Comparison of multiplex PCR, enzyme immunoassay and cell culture methods for the detection of enterotoxinogenic Bacillus cereus Fulltext Access 6 Pages 2009
A simple modification of a silicic acid lipid fractionation protocol to eliminate free fatty acids from glycolipid and phospholipid fractions Fulltext Access 6 Pages 2009
The production of recombinant human laminin-332 in a Leishmania tarentolae expression system Fulltext Access 6 Pages 2009
Chaperone-assisted production of active human Rab8A GTPase in Escherichia coli Fulltext Access 6 Pages 2009
Preparation of isotopically labelled recombinant β-defensin for NMR studies Fulltext Access 6 Pages 2009
Soluble expression and one-step purification of a neurotoxin Huwentoxin-I in Escherichia coli Fulltext Access 6 Pages 2009
An optimized procedure for efficient phage display of antibody fragments with a low folding efficiency Fulltext Access 6 Pages 2009
Expression of Epstein-Barr virus EBNA1 protein in Escherichia coli: Purification under nondenaturing conditions and use in DNA-binding studies Fulltext Access 6 Pages 2009
Expression of a Trichoderma reesei β-xylanase gene in Escherichia coli and activity of the enzyme on fiber-bound substrates Fulltext Access 6 Pages 2009
Heterologous expression and molecular characterization of the NAD(P)H:acceptor oxidoreductase (FerB) of Paracoccus denitrificans Fulltext Access 6 Pages 2009
Over-expression in E. coli and purification of the human OCTN1 transport protein Fulltext Access 6 Pages 2009
One-step expression and purification of single-chain variable antibody fragment using an improved hexahistidine tag phagemid vector Fulltext Access 6 Pages 2009
Cloning, production, purification and preliminary crystallographic analysis of a glycosidase from the food lactic acid bacterium Lactobacillus plantarum CECT 748T Fulltext Access 6 Pages 2009
Secretory expression and characterization of a highly Ca2+-activated thermostable L2 lipase Fulltext Access 6 Pages 2009
High-level expression and characterization of Fusarium solani cutinase in Pichia pastoris Fulltext Access 6 Pages 2009
Expression, purification and characterization of aprotinin and a human analogue of aprotinin Fulltext Access 6 Pages 2009
High throughput purification of recombinant human growth hormone using radial flow chromatography Fulltext Access 6 Pages 2009
Bacterial expression of a eukaryotic membrane protein in fusion to various Mistic orthologs Fulltext Access 6 Pages 2009
Cell-free synthesis and combinatorial selective 15N-labeling of the cytotoxic protein amoebapore A from Entamoeba histolytica Fulltext Access 6 Pages 2009
Expression, purification and characterization of a functional extracellular domain of porcine FcγRII Fulltext Access 6 Pages 2009
Recombinant human serine racemase: Enzymologic characterization and comparison with its mouse ortholog Fulltext Access 6 Pages 2009
Expression and functional characterization of the plant antimicrobial snakin-1 and defensin recombinant proteins Fulltext Access 6 Pages 2009
MEP chromatography of antibody and Fc-fusion protein using aqueous arginine solution Fulltext Access 6 Pages 2009
Pilot-scale fermentation, purification, and characterization of recombinant human Oncostatin M in Pichia pastoris Fulltext Access 6 Pages 2009
Expression, purification and osteogenic bioactivity of recombinant human BMP-4, -9, -10, -11 and -14 Fulltext Access 6 Pages 2009
Refolding and purification of the human secreted group IID phospholipase A2 expressed as inclusion bodies in Escherichia coli Fulltext Access 6 Pages 2009
Metabolic state, neurohormones, and vagal stimulation, not increased serotonin, orchestrate postprandial drowsiness Fulltext Access 6 Pages 2009
Universal and rapid method for purification of GFP-like proteins by the ethanol extraction Fulltext Access 6 Pages 2009
Transient expression and purification of chimeric heavy chain antibodies Fulltext Access 6 Pages 2009
A dual-functional E. coli vector for expressing recombinant protein with high solubility and antigen presentation ability Fulltext Access 6 Pages 2009
Expression and purification of the calcium binding photoprotein mitrocomin using ZZ-domain as a soluble partner in E. coli cells Fulltext Access 6 Pages 2009
Production of the p24 capsid protein from HIV-1 subtype C in Arabidopsis thaliana and Daucus carota using an endoplasmic reticulum-directing SEKDEL sequence in protein expression constructs Fulltext Access 6 Pages 2009
High-yield production and characterization of biologically active recombinant aprotinin expressed in Saccharomyces cerevisiae Fulltext Access 6 Pages 2009
Expression and purification of recombinant M-Pol I from Saccharomyces cerevisiae with α-1,6 mannosylpolymerase activity Fulltext Access 6 Pages 2009
Improved production of holotoxin Stx2 with biological activities by using a single-promoter vector and an auto-induction expression system Fulltext Access 6 Pages 2009
A unique post-translational processing of an exo-β-1,3-glucanase of Penicillium sp. KH10 expressed in Aspergillus oryzae Fulltext Access 6 Pages 2009
Purification and characterization of a novel ribosome-inactivating protein from seeds of Trichosanthes kirilowii Maxim Fulltext Access 6 Pages 2009
Cloning, expression and characterization of recombinant elastase from Pseudomonas aeruginosa in Picha pastoris Fulltext Access 6 Pages 2009
Effect of homologous serotonin receptor loop substitutions on the heterologous expression in Pichia of a chimeric acetylcholine-binding protein with α-bungarotoxin-binding activity Fulltext Access 6 Pages 2009
Purification and characterisation of the breast cancer metastasis suppressor, BRMS1 Fulltext Access 6 Pages 2009
Factors affecting endotoxin removal from recombinant therapeutic proteins by anion exchange chromatography Fulltext Access 6 Pages 2009
Production and purification of the isolated family 2a carbohydrate-binding module from Cellulomonas fimi Fulltext Access 6 Pages 2009
Expression and purification of isotopically labeled peptide inhibitors and substrates of cAMP-dependant protein kinase A for NMR analysis Fulltext Access 6 Pages 2009
TrxA mediating fusion expression of antimicrobial peptide CM4 from multiple joined genes in Escherichia coli Fulltext Access 6 Pages 2009
High yield bacterial expression and purification of active recombinant PA28αβ complex Fulltext Access 6 Pages 2009
Cloning, expression, characterization and crystallization of BRP39, a signalling glycoprotein expressed during mammary gland apoptosis Fulltext Access 6 Pages 2009
The effect of the cell death suppressor vMIA on the production of a recombinant protein in the adenovirus-293 expression system Fulltext Access 6 Pages 2009
Purification, characterization and crystallization of pyrroline-5-carboxylate reductase from the hyperthermophilic archeon Sulfolobus Solfataricus Fulltext Access 6 Pages 2009
CBM21 starch-binding domain: A new purification tag for recombinant protein engineering Fulltext Access 6 Pages 2009