A mutation in human VAP-B–MSP domain, present in ALS patients, affects the interaction with other cellular proteins Fulltext Access 8 Pages 2007
Cloning and overexpression of antifungal barley chitinase gene in Escherichia coli Fulltext Access 8 Pages 2007
Preparation and characterization of recombinant protein ScFv(CD11c)-TRP2 for tumor therapy from inclusion bodies in Escherichia coli Fulltext Access 8 Pages 2007
Increased yield of high purity recombinant human interferon-γ utilizing reversed phase column chromatography Fulltext Access 8 Pages 2007
Expression, purification, characterization and crystallization of a recombinant human cytosolic β-glucosidase produced in insect cells Fulltext Access 8 Pages 2007
Helicobacter pylori acyl carrier protein: Expression, purification, and its interaction with β-hydroxyacyl-ACP dehydratase Fulltext Access 8 Pages 2007
Expression, purification and characterization of calcium-triggered luciferin-binding protein of Renilla reniformis Fulltext Access 8 Pages 2007
An improved strategy for high-level production of TEV protease in Escherichia coli and its purification and characterization Fulltext Access 8 Pages 2007
Molecular cloning and functional expression of bovine deoxyhypusine hydroxylase cDNA and homologs Fulltext Access 8 Pages 2007
Functional expression of the keratinolytic serine protease gene sfp2 from Streptomyces fradiae var. k11 in Pichia pastoris Fulltext Access 8 Pages 2007
Expression, purification and characterization of full-length RNA-free hepatitis B core particles Fulltext Access 8 Pages 2007
High-level expression of the recombinant hybrid peptide cecropinA(1-8)–magainin2(1-12) with an ubiquitin fusion partner in Escherichia coli Fulltext Access 8 Pages 2007
Overexpression and purification of human calcitonin gene-related peptide–receptor component protein in Escherichia coli Fulltext Access 8 Pages 2007
Immunoaffinity purification and characterization of RNA polymerase from Shewanella oneidensis Fulltext Access 8 Pages 2007
Time- and temperature-dependent acetylation of the chemokine RANTES produced in recombinant Escherichia coli Fulltext Access 8 Pages 2007
Heterologous expression and characterization of the recombinant bradykinin B2 receptor using the methylotrophic yeast Pichia pastoris Fulltext Access 8 Pages 2007
Profiling of membrane protein variants in a baculovirus system by coupling cell-surface detection with small-scale parallel expression Fulltext Access 8 Pages 2007
Increasing the expression levels of papillomavirus major capsid protein in Escherichia coli by N-terminal deletion Fulltext Access 8 Pages 2007
The AviD-tag, a NeutrAvidin/avidin specific peptide affinity tag for the immobilization and purification of recombinant proteins Fulltext Access 8 Pages 2007
Purification and refolding optimization of recombinant bovine enterokinase light chain overexpressed in Escherichia coli Fulltext Access 8 Pages 2007
Evaluation of different culture conditions for high-level soluble expression of human cyclin A2 with pET vector in BL21 (DE3) and spectroscopic characterization of its inclusion body structure Fulltext Access 8 Pages 2007
Molecular cloning and biochemical characterization of Leishmania donovani serine hydroxymethyltransferase Fulltext Access 8 Pages 2007
Refolding, purification, and activation of miniplasminogen and microplasminogen isolated from E. coli inclusion bodies Fulltext Access 8 Pages 2007
Expression and purification of milligram levels of inactive G-protein coupled receptors in E. coli Fulltext Access 8 Pages 2007
‘Candidatus Rhabdochlamydia crassificans’, an intracellular bacterial pathogen of the cockroach Blatta orientalis (Insecta: Blattodea) Fulltext Access 8 Pages 2007
New vectors for co-expression of proteins: Structure of Bacillus subtilis ScoAB obtained by high-throughput protocols Fulltext Access 8 Pages 2007
Expression, purification, and in vitro cysteine-10 modification of native sequence recombinant human transthyretin Fulltext Access 8 Pages 2007
A biologically active angiogenesis inhibitor, human serum albumin-TIMP-2 fusion protein, secreted from Saccharomyces cerevisiae Fulltext Access 8 Pages 2007
Expression of the nucleocytoplasmic tobacco lectin in the yeast Pichia pastoris Fulltext Access 8 Pages 2007
Purification and characterization of recombinant extracellular domain of human HER2 from Escherichia coli Fulltext Access 8 Pages 2007
Cloning, expression, purification, and characterization of a novel epoxide hydrolase from Aspergillus niger SQ-6 Fulltext Access 8 Pages 2007
Efficiency of fluorescence in situ hybridization for bacterial cell identification in temporary river sediments with contrasting water content Fulltext Access 8 Pages 2007
Molecular typing of wine yeast strains Saccharomyces bayanus var. uvarum using microsatellite markers Fulltext Access 8 Pages 2007
Molecular identification and differentiation of Brevibacterium species and strains Fulltext Access 8 Pages 2007
Halomonas alkaliantarctica sp. nov., isolated from saline lake Cape Russell in Antarctica, an alkalophilic moderately halophilic, exopolysaccharide-producing bacterium Fulltext Access 8 Pages 2007
Subspecies-specific distribution of intervening sequences in the Bacillus subtilis prophage ribonucleotide reductase genes Fulltext Access 8 Pages 2007
Distribution of three genes involved in the PJM1 iron-sequestering system in various Vibrio anguillarum serogroups Fulltext Access 8 Pages 2007
Functional expression and refolding of new alkaline esterase, EM2L8 from deep-sea sediment metagenome Fulltext Access 8 Pages 2007
Novel facultative anaerobic acidotolerant Telmatospirillum siberiense gen. nov. sp. nov. isolated from mesotrophic fen Fulltext Access 8 Pages 2007
Evolutionary diversity of ketoacyl synthases in cellulolytic myxobacterium Sorangium Fulltext Access 8 Pages 2007
New Afipia and Bosea strains isolated from various water sources by amoebal co-culture Fulltext Access 8 Pages 2007
Identification, cloning, expression, and characterization of a highly thermostable single-stranded-DNA-binding protein (SSB) from Deinococcus murrayi Fulltext Access 8 Pages 2007
Production of recombinant human α1-microglobulin and mutant forms involved in chromophore formation Fulltext Access 8 Pages 2007
High-level expression of a soluble and functional fibronectin type II domain from MMP-2 in the Escherichia coli cytoplasm for solution NMR studies Fulltext Access 8 Pages 2007
Comparison of natural and recombinant clitocypins, the fungal cysteine protease inhibitors Fulltext Access 8 Pages 2007
Expression, purification, and characterization of human acetyl-CoA carboxylase 2 Fulltext Access 8 Pages 2007
Prokaryotic expression and polyclonal antibody preparation of a novel Rab-like protein mRabL5 Fulltext Access 8 Pages 2007
On-column refolding of recombinant chemokines for NMR studies and biological assays Fulltext Access 8 Pages 2007
Optimisation of production of a domoic acid-binding scFv antibody fragment in Escherichia coli using molecular chaperones and functional immobilisation on a mesoporous silicate support Fulltext Access 8 Pages 2007
In situ multi-wavelength fluorescence spectroscopy as effective tool to simultaneously monitor spore germination, metabolic activity and quantitative protein production in recombinant Aspergillus niger fed-batch cultures Fulltext Access 8 Pages 2007
Isolation and characterization of a metagenome-derived and cold-active lipase with high stereospecificity for (R)-ibuprofen esters Fulltext Access 8 Pages 2007
Pattern of anti-HIV dipyranocoumarin expression in callus cultures of Calophyllum inophyllum Linn. Fulltext Access 8 Pages 2007
Expression of thermostable microbial cellulases in the chloroplasts of nicotine-free tobacco Fulltext Access 8 Pages 2007
Circumventing the heterogeneity and instability of human serum albumin-interferon-α2b fusion protein by altering its orientation Fulltext Access 8 Pages 2007
Properties of a chimeric glucose dehydrogenase improved by site directed mutagenesis Fulltext Access 8 Pages 2007
The histone deacetylase inhibitor sodium butyrate inhibits baculovirus-mediated transgene expression in Sf9 cells Fulltext Access 8 Pages 2007
Substrate specificity of a galactose 6-phosphate isomerase from Lactococcus lactis that produces d-allose from d-psicose Fulltext Access 8 Pages 2007
Prevention of LLC-PK1 cell overgrowth in a bioartificial renal tubule device using a MEK inhibitor, U0126 Fulltext Access 8 Pages 2007
Bioproduction of p-hydroxybenzoate from renewable feedstock by solvent-tolerant Pseudomonas putida S12 Fulltext Access 8 Pages 2007
Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of activity and enantioselectivity of the enzyme by protein engineering Fulltext Access 8 Pages 2007
The influence of circulation frequency on fungal morphology: A case study considering Kolmogorov microscale in constant specific energy dissipation rate cultures of Trichoderma harzianum Fulltext Access 8 Pages 2007
Simultaneous biosynthesis of (+)-geodin by a lovastatin-producing fungus Aspergillus terreus Fulltext Access 8 Pages 2007
Recovery of succinic acid produced by fermentation of a metabolically engineered Mannheimia succiniciproducens strain Fulltext Access 8 Pages 2007
Hybrid semi-parametric mathematical systems: Bridging the gap between systems biology and process engineering Fulltext Access 8 Pages 2007
An efficient method for protein phosphorylation using the artificially introduced of cognate-binding modules into kinases and substrates Fulltext Access 8 Pages 2007
Directed evolution of transketolase activity on non-phosphorylated substrates Fulltext Access 8 Pages 2007
Characterization of the unique organization and co-regulation of a gene cluster required for phenol and benzene catabolism in Pseudomonas sp. M1 Fulltext Access 8 Pages 2007
Redirecting metabolic fluxes through cofactor engineering: Role of CoA-esters pool during l(−)-carnitine production by Escherichia coli Fulltext Access 8 Pages 2007
Large-scale production and efficient recovery of PHB with desirable material properties, from the newly characterised Bacillus cereus SPV Fulltext Access 8 Pages 2007
A novel variant of Thermotoga neapolitana β-glucosidase B is an efficient catalyst for the synthesis of alkyl glucosides by transglycosylation Fulltext Access 8 Pages 2007
The dodecameric vanadium-dependent haloperoxidase from the marine algae Corallina officinalis: Cloning, expression, and refolding of the recombinant enzyme Fulltext Access 8 Pages 2007
Suitability of yeast- and Escherichia coli-expressed hepatitis B virus core antigen derivatives for detection of anti-HBc antibodies in human sera Fulltext Access 8 Pages 2007
Overexpression, purification and characterization of the catalytic component of Oplophorus luciferase in the deep-sea shrimp, Oplophorus gracilirostris Fulltext Access 8 Pages 2007
Stability engineering, biophysical, and biological characterization of the myeloid activating receptor immunoglobulin-like transcript 1 (ILT1/LIR-7/LILRA2) Fulltext Access 8 Pages 2007
Recombinant thymosin beta 4 can promote full-thickness cutaneous wound healing Fulltext Access 8 Pages 2007
Expression and purification of the cancer antigen SSX2: A potential cancer vaccine Fulltext Access 8 Pages 2007
High-yield expression and purification of isotopically labeled norcoclaurine synthase, a Bet v 1-homologous enzyme, from Thalictrum flavum for NMR studies Fulltext Access 8 Pages 2007
Biophysical and biochemical characterization of reconstituted and purified Rhodobacter sphaeroides cytochrome c oxidase in phospholipid vesicles sheds insight into its functional oligomeric structure Fulltext Access 8 Pages 2007
Enhanced production of secretory β1,3-N-acetylglucosaminyltransferase 2 fusion protein into hemolymph of Bombyx mori larvae using recombinant BmNPV bacmid integrated signal sequence Fulltext Access 8 Pages 2007
Monoclonal antibody against non-dominant epitopes of HBV e Ag was raised by antigen–antibody co-immunization Fulltext Access 8 Pages 2007
An siRNA-based system for differential regulation of ectopic gene expression constructs Fulltext Access 8 Pages 2007
The inherent property of polyhydroxyalkanoate synthase to form spherical PHA granules at the cell poles: The core region is required for polar localization Fulltext Access 8 Pages 2007
Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris Fulltext Access 8 Pages 2007
Calorimetric bioprocess monitoring by small modifications to a standard bench-scale bioreactor Fulltext Access 8 Pages 2007
Characterization of the proteins of bacterial strain isolated from contaminated site involved in heavy metal resistance—A proteomic approach Fulltext Access 8 Pages 2007
Rational design of novel mutants of fungal 17β-hydroxysteroid dehydrogenase Fulltext Access 8 Pages 2007
Entrapment in E. coli improves the operational stability of recombinant β-glycosidase CelB from Pyrococcus furiosus and facilitates biocatalyst recovery Fulltext Access 8 Pages 2007
Application of enzymes, sodium tripolyphosphate and cation exchange resin for the release of extracellular polymeric substances from sewage sludge: Characterization of the extracted polysaccharides/glycoconjugates by a panel of lectins Fulltext Access 8 Pages 2007
A highly stable cambialistic-superoxide dismutase from Antrodia camphorata: Expression in yeast and enzyme properties Fulltext Access 8 Pages 2007
Collagen vitrigel membrane useful for paracrine assays in vitro and drug delivery systems in vivo Fulltext Access 8 Pages 2007
Post-transcriptional regulatory element boosts baculovirus-mediated gene expression in vertebrate cells Fulltext Access 8 Pages 2007
Biotransformation of isoeugenol to vanillin by a newly isolated Bacillus pumilus strain: Identification of major metabolites Fulltext Access 8 Pages 2007
Role of the plasma membrane ROS-generating NADPH oxidase in CD34+ progenitor cells preservation by hypoxia Fulltext Access 8 Pages 2007