FISH and chips: Marine bacterial communities analyzed by flow cytometry based on microfluidics Fulltext Access 9 Pages 2006
Comparison of methods and animal models commonly used for investigation of fecal microbiota: Effects of time, host and gender Fulltext Access 9 Pages 2006
rpoB gene: A target for identification of LAB cocci by PCR-DGGE and melting curves analyses in real time PCR Fulltext Access 9 Pages 2006
Detection of Clavibacter michiganensis subsp. michiganensis in tomato seeds using immunomagnetic separation Fulltext Access 9 Pages 2006
A fermentation assay to evaluate the effectiveness of antimicrobial agents on gut microflora Fulltext Access 9 Pages 2006
Quantification of Vibrio penaeicida, the etiological agent of Syndrome 93 in New Caledonian shrimp, by real-time PCR using SYBR Green I chemistry Fulltext Access 9 Pages 2006
Rapid laboratory detection of meningococcal disease outbreaks caused by serogroup C Neisseria meningitidis Fulltext Access 9 Pages 2006
Bioluminescence of Vibrio fischeri in continuous culture: Optimal conditions for stability and intensity of photoemission Fulltext Access 9 Pages 2006
Quantification of homogentisate-1,2-dioxygenase expression in a fungus degrading ethylbenzene Fulltext Access 9 Pages 2006
Rapid screening of the fermentation profiles of wine yeasts by Fourier transform infrared spectroscopy Fulltext Access 9 Pages 2006
Improved expression and purification of the correctly folded response regulator PlnC from lactobacilli Fulltext Access 9 Pages 2006
Laboratory-based simulation of freezing profiles of beef trim for Escherichia coli O157 survival determinations Fulltext Access 9 Pages 2006
Enhancement of protein synthesis by an inorganic polyphosphate in an E. coli cell-free system Fulltext Access 9 Pages 2006
Rapid detection and species identification of Mycobacterium spp. using real-time PCR and DNA-Microarray Fulltext Access 9 Pages 2006
An improved method for the selective detection of fungi in hospital waters by solid phase cytometry Fulltext Access 9 Pages 2006
Real-time PCR assay to quantify Fusarium graminearum wild-type and recombinant mutant DNA in plant material Fulltext Access 9 Pages 2006
An AFLP-based database of Shigella flexneri and Shigella sonnei isolates and its use for the identification of untypable Shigella strains Fulltext Access 9 Pages 2006
A novel system of genetic transformation allows multiple integrations of a desired gene in Saccharomyces cerevisiae chromosomes Fulltext Access 9 Pages 2006
Site-directed gene replacement of the phytopathogen Xanthomonas axonopodis pv. citri Fulltext Access 9 Pages 2006
Confidence intervals of similarity values determined for cloned SSU rRNA genes from environmental samples Fulltext Access 9 Pages 2006
Comparison of three ELISA protocols to measure antibody responses elicited against serogroup C meningococcal polysaccharide in mouse, monkey and human sera Fulltext Access 9 Pages 2006
Description and critical appraisal of principal components analysis (PCA) methodology applied to pulsed-field gel electrophoresis profiles of methicillin-resistant Staphylococcus aureus isolates Fulltext Access 9 Pages 2006
A rapid method combining immunofluorescence and flow cytometry for improved understanding of competitive interactions between lactic acid bacteria (LAB) and methicillin-resistant S. aureus (MRSA) in mixed culture Fulltext Access 9 Pages 2006
Detection of Fusarium culmorum in wheat by a surface plasmon resonance-based DNA sensor Fulltext Access 9 Pages 2006
A detailed phenotypic and genotypic description of Pseudomonas strain OX1 Fulltext Access 9 Pages 2006
The association between anatomic site of Candida colonization, invasive candidiasis, and mortality in critically ill surgical patients Fulltext Access 9 Pages 2006
Concentrations in plasma, urinary excretion and bactericidal activity of levofloxacin (500 mg) versus ciprofloxacin (500 mg) in healthy volunteers receiving a single oral dose Fulltext Access 9 Pages 2006
Mouse salivary glands and human β-defensin-2 as a study model for antimicrobial gene therapy: technical considerations Fulltext Access 9 Pages 2006
Effects of sustained antibiotic bactericidal treatment on Chlamydia trachomatis-infected epithelial-like cells (HeLa) and monocyte-like cells (THP-1 and U-937) Fulltext Access 9 Pages 2006
Heterogeneous resistance to vancomycin in Staphylococcus epidermidis, Staphylococcus haemolyticus and Staphylococcus warneri clinical strains: characterisation of glycopeptide susceptibility profiles and cell wall thickening Fulltext Access 9 Pages 2006
Characterisation of antimicrobial resistance patterns and class 1 integrons among Escherichia coli and Salmonella enterica serovar Choleraesuis strains isolated from humans and swine in Taiwan Fulltext Access 9 Pages 2006
Prevalence and antimicrobial susceptibility patterns among gastroenteritis-causing pathogens recovered in Europe and Latin America and Salmonella isolates recovered from bloodstream infections in North America and Latin America: report from the SENTRY Ant Fulltext Access 9 Pages 2006
Contemporary antimicrobial activity of triple antibiotic ointment: a multiphased study of recent clinical isolates in the United States and Australia Fulltext Access 9 Pages 2006
Analysis of Salmonella spp. with resistance to extended-spectrum cephalosporins and fluoroquinolones isolated in North America and Latin America: report from the SENTRY Antimicrobial Surveillance Program (1997–2004) Fulltext Access 9 Pages 2006
Rapid identification of fungal pathogens in BacT/ALERT, BACTEC, and BBL MGIT media using polymerase chain reaction and DNA sequencing of the internal transcribed spacer regions Fulltext Access 9 Pages 2006
Prospective evaluation of the treatment and outcome of community-acquired pneumonia according to the Pneumonia Severity Index in VHA hospitals Fulltext Access 9 Pages 2006
Clonal occurrences of multidrug-resistant Gram-negative bacilli: report from the Meropenem Yearly Susceptibility Test Information Collection Surveillance Program in the United States (2004) Fulltext Access 9 Pages 2006
Characterization of fluoroquinolone-resistant β-hemolytic Streptococcus spp. isolated in North America and Europe including the first report of fluoroquinolone-resistant Streptococcus dysgalactiae subspecies equisimilis: Report from the SENTRY Antimicrobi Fulltext Access 9 Pages 2006
Evaluation of gene expression analysis using RNA-targeted partial genome arrays Fulltext Access 9 Pages 2006
Development of a polymerase chain reaction assay for the specific identification of Burkholderia mallei and differentiation from Burkholderia pseudomallei and other closely related Burkholderiaceae Fulltext Access 9 Pages 2006
Utility of an immunocapture-agglutination test and an enzyme-linked immunosorbent assay test against cytosolic proteins from Brucella melitensis B115 in the diagnosis and follow-up of human acute brucellosis Fulltext Access 9 Pages 2006
Cephalosporin resistance in Klebsiella pneumoniae from Nova Scotia, Canada Fulltext Access 9 Pages 2006
Infrequent MODS TB culture cross-contamination in a high-burden resource-poor setting Fulltext Access 9 Pages 2006
Cytokine gene polymorphisms in Colombian patients with different clinical presentations of tuberculosis Fulltext Access 9 Pages 2006
Lymphadenitis as a major element of disease in the guinea pig model of tuberculosis Fulltext Access 9 Pages 2006
Characterization of the immunological features of tuberculous pericardial effusions in HIV positive and HIV negative patients in contrast with non-tuberculous effusions Fulltext Access 9 Pages 2006
Changes in microbial and soil properties following amendment with treated and untreated olive mill wastewater Fulltext Access 9 Pages 2006
Expression of LASS2 controlled by LAG1 or ADH1 promoters cannot functionally complement Lag1p Fulltext Access 9 Pages 2006
Rapid identification of bacteria by real-time amplification and sequencing of the 16S rRNA gene Fulltext Access 9 Pages 2006
Primers containing universal bases reduce multiple amoA gene specific DGGE band patterns when analysing the diversity of beta-ammonia oxidizers in the environment Fulltext Access 9 Pages 2006
Expression and characterization of recombinant tyramine β-monooxygenase from Drosophila: A monomeric copper-containing hydroxylase Fulltext Access 9 Pages 2006
Expression and purification of human placenta lactogen in Escherichia coli Fulltext Access 9 Pages 2006
Cloning, expression, purification, and characterization of zebrafish cytosolic serine hydroxymethyltransferase Fulltext Access 9 Pages 2006
Expression and functional purification of a glycosylation deficient version of the human adenosine 2a receptor for structural studies Fulltext Access 9 Pages 2006
Method for large-scale isolation and purification of R-phycoerythrin from red alga Polysiphonia urceolata Grev Fulltext Access 9 Pages 2006
High-level expression and characterization of Galactomyces geotrichum (BT107) lipase I in Pichia pastoris Fulltext Access 9 Pages 2006
Purification, crystallization and preliminary X-ray diffraction analysis of the phage T4 vertex protein gp24 and its mutant forms Fulltext Access 9 Pages 2006
Use of ribosomal promoters from Burkholderia cenocepacia and Burkholderia cepacia for improved expression of transporter protein in Escherichia coli Fulltext Access 9 Pages 2006
Cerato-platanin, a phytotoxic protein from Ceratocystis fimbriata: Expression in Pichia pastoris, purification and characterization Fulltext Access 9 Pages 2006
Coexpression of the subunits of T7 DNA polymerase from an artificial operon allows one-step purification of active gp5/Trx complex Fulltext Access 9 Pages 2006
Codon optimization, expression, and characterization of an internalizing anti-ErbB2 single-chain antibody in Pichia pastoris Fulltext Access 9 Pages 2006
Expression and purification of the subunits of human translational initiation factor 2 (eIF2): Phosphorylation of eIF2α and β Fulltext Access 9 Pages 2006
Recombinant production of anti-HIV protein, griffithsin, by auto-induction in a fermentor culture Fulltext Access 9 Pages 2006
Cloning, bacterial expression, purification and structural characterization of N-terminal-repetitive domain of γ-Gliadin Fulltext Access 9 Pages 2006
Large-scale preparation of biologically active mouse and rat leptins and their L39A/D40A/F41A muteins which act as potent antagonists Fulltext Access 9 Pages 2006
Construction, expression, and purification of HIV–TAT-survivin (T34A) mutant: A pro-apoptosis protein in Escherichia coli Fulltext Access 9 Pages 2006
Automated purification of recombinant proteins: Combining high-throughput with high yield Fulltext Access 9 Pages 2006
Expression, purification, and characterization of a new heterotetramer structure of leucyl-tRNA synthetase from Aquifex aeolicus in Escherichia coli Fulltext Access 9 Pages 2006
High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system Fulltext Access 9 Pages 2006
Purification and characterization of Mycobacterium tuberculosis 1d-myo-inosityl-2-acetamido-2-deoxy-α-d-glucopyranoside deacetylase, MshB, a mycothiol biosynthetic enzyme Fulltext Access 9 Pages 2006
Expression optimization and purification process development of an engineered soluble recombinant mouse linker of activation of T cells using surface enhanced laser desorption/ionization-mass spectrometry Fulltext Access 9 Pages 2006
Overexpression and characterization of two human salivary proline rich proteins Fulltext Access 9 Pages 2006
An expression vector tailored for large-scale, high-throughput purification of recombinant proteins Fulltext Access 9 Pages 2006
Purification and characterization of the chaperone-like Hsp26 from Saccharomyces cerevisiae Fulltext Access 9 Pages 2006
Chemical ligation and cleavage on solid support facilitate recombinant peptide purification Fulltext Access 9 Pages 2006
Purification and scale-up of a recombinant heavy chain fragment C of botulinum neurotoxin serotype E in Pichia pastoris GS115 Fulltext Access 9 Pages 2006
Evaluation of amplified ribosomal DNA restriction analysis (ARDRA) and species-specific PCR for identification of Bifidobacterium species Fulltext Access 9 Pages 2006
Distribution of F- and A/V-type ATPases in Thermus scotoductus and other closely related species Fulltext Access 9 Pages 2006
Application of SSCP–PCR fingerprinting to profile the yeast community in raw milk Salers cheeses Fulltext Access 9 Pages 2006
Occurrence of toxigenic cyanobacterial blooms in freshwaters of Sri Lanka Fulltext Access 9 Pages 2006
Chimaereicella alkaliphila gen. nov., sp. nov., a Gram-negative alkaliphilic bacterium isolated from a nonsaline alkaline groundwater Fulltext Access 9 Pages 2006
PCR-based strategy to detect contamination with mycotoxigenic Fusarium species in maize Fulltext Access 9 Pages 2006
Optimization of the primary recovery of human interferon α2b from Escherichia coli inclusion bodies Fulltext Access 9 Pages 2006
In vitro activation, purification, and characterization of Escherichia coli expressed aryl-alcohol oxidase, a unique H2O2-producing enzyme Fulltext Access 9 Pages 2006
Refolding, purification, and characterization of human and murine RegIII proteins expressed in Escherichia coli Fulltext Access 9 Pages 2006
Two methods for improved purification of full-length mammalian proteins that have poor expression and/or solubility using standard Escherichia coli procedures Fulltext Access 9 Pages 2006
cDNA cloning, expression, purification, distribution, and characterization of biologically active canine alanine aminotransferase-1 Fulltext Access 9 Pages 2006
A novel lysozyme from Xanthomonas oryzae phage ÏXo411 active against Xanthomonas and Stenotrophomonas Fulltext Access 9 Pages 2006
Semliki Forest virus vectors for overexpression of 101 G protein-coupled receptors in mammalian host cells Fulltext Access 9 Pages 2006
Screening and characterization of a novel esterase from a metagenomic library Fulltext Access 9 Pages 2006
Design and optimization of a recombinant system for large-scale production of the MPT64 antigen from Mycobacterium tuberculosis Fulltext Access 9 Pages 2006
The development of an efficient system for heterologous expression of cytochrome P450s in Escherichia coli using hemA gene co-expression Fulltext Access 9 Pages 2006
Co-expression of multiple subunits enables recombinant SNAPC assembly and function for transcription by human RNA polymerases II and III Fulltext Access 9 Pages 2006
Cloning, expression and rapid purification of active recombinant mycothiol ligase as B1 immunoglobulin binding domain of streptococcal protein G, glutathione-S-transferase and maltose binding protein fusion proteins in Mycobacterium smegmatis Fulltext Access 9 Pages 2006
Bacterial production of biologically active canine interleukin-1β by seamless SUMO tagging and removal Fulltext Access 9 Pages 2006
Rare codon clusters at 5′-end influence heterologous expression of archaeal gene in Escherichia coli Fulltext Access 9 Pages 2006
An improved recombinant mammalian cell expression system for human transforming growth factor-β2 and -β3 preparations Fulltext Access 9 Pages 2006
Functional shikimate dehydrogenase from Mycobacterium tuberculosis H37Rv: Purification and characterization Fulltext Access 9 Pages 2006