Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10593527 | Bioorganic & Medicinal Chemistry Letters | 2013 | 5 Pages |
Abstract
A novel N-acetylglucosamine-1-phosphate pyrophosphorylase was identified from Campylobacter jejuni NCTC 11168. An unprecedented degree of substrate promiscuity has been revealed by systematic studies on its substrate specificities towards sugar-1-P and NTP. The yields of the synthetic reaction of seven kinds of sugar nucleotides catalyzed by the enzyme were up to 60%. In addition, the yields of the other nine were around 20%. With this enzyme, three novel sugar nucleotide analogs were synthesized on a preparative scale and well characterized.
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Authors
Junqiang Fang, Mengyang Xue, Guofeng Gu, Xian-wei Liu, Peng George Wang,