Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10971821 | Fish & Shellfish Immunology | 2015 | 23 Pages |
Abstract
In order to explore the potential roles of microRNAs (miRNAs) in regulating Toll-like receptor (TLR) pathways, we identified Toll interacting protein as a putative target of miR-200 in Apostichopus japonicus coelomocytes by RNA-seq screening (denoted as AjTollip). The positive expression profiles of miR-200 and AjTollip were detected in both LPS exposure primary coelomocytes and Vibrio splendidus challenge sea cucumber. Co-infection miR-200 mimics significantly elevated the expression of AjTollip and its down-stream molecules. In contrast, miR-200 inhibitor significantly repressed the expression of these TLR-pathway members. More importantly, miR-200 displayed not only to enhance coelomocytes antibacterial activities, but to suppress LPS priming in vitro. Overall, all these results will enhance our understanding on miR-200 regulatory roles in anti-bacterial process in sea cucumber.
Keywords
LPSinterleukin-1 receptor-associated kinasesForkhead box A2MIP2LBP/BPImiR-200BZLF1FOXA2ZEB1/2CFUTLRIL-6IκBCXCL1qPCRSUSQuantitative PCRtoll like receptorRISCTollipSkin ulceration syndromeIRAK-1Antibacterial activitylipopolysaccharideRNA-Induced Silencing Complexinhibitor of NF-κBchemokine (C-X-C motif) ligand 1
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Authors
Zhimeng Lv, Chenghua Li, Pengjuan Zhang, Zhenhui Wang, Weiwei Zhang, Chun-Hua Jin,