Particularity and universality of a putative Gram-negative bacteria-binding protein (GNBP) gene from amphioxus (Branchiostoma belcheri): Insights into the function and evolution of GNBP

► AmphiGNBP gene was cloned from amphioxus with a 1784 bp cDNA transcription product. ► AmphiGNBP encodes two extra WSC domains. ► AmphiGNBP might undergo intensive domain shuffling during the Cambrian explosion. ► AmphiGNBP was mainly expressed in hepatic cecum and intestine. ► AmphiGNBP was up-regulated after LPS stimulation.