Activation free energy of Zn(II), Co(II) binding to metallo-β-lactamase ImiS

In an effort to understand the recombination of a B2 metallo-β  -lactamase (MβL), the binding of metals to apo-ImiS was studied by isothermal titration calorimetry and fluorescence spectra. The binding of Zn(II), Co(II) to apo-ImiS resulted in activation free energies ΔG≠θ values of 93.719 and 92.948 kJ mol−1, respectively, and increasing of fluorescence intensity at maxima emission of 340 nm.

Graphical abstractBinding of Zn(II), Co(II) to apo-ImiS results in an activation free energy ΔG≠θ value of 92.948 and 93.908 kJ mol−1, respectively, and increasing of fluorescence intensity at maxima emission of 340 nm.Figure optionsDownload full-size imageDownload as PowerPoint slide