Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1255162 | Chinese Chemical Letters | 2012 | 4 Pages |
Abstract
Detection for deoxyribozyme (DNAzyme) cleavage usually needs complex and time-consuming radial labeling, gel electrophoresis and autoradiography. A new approach was reported for detection DNAzyme cleavage product based on molecular beacon (MB). Part of the loop of MB was designed to complementary to DNAzyme cleavage product. MB was employed to monitor ligation process of RNA/DNA complex and to convert directly cleavage product information into fluorescence signal. Detection limit of the assay is 0.02 nmol/L. The cleavage product of 8−17 DNAzyme against HCV-RNA was detected perfectly based on this assay. The method is fast, simple and ultrasensitive, which might hold great promise in DNAzyme reaction and DNAzyme gene therapy.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Hong Peng Xie, Xiang Xian Meng, Hongyan Su, Qing Yun Cai, Yong Jun Tan, Xiao Qin Huang,