Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1256397 | Chinese Chemical Letters | 2007 | 4 Pages |
Abstract
An ss-DNA gold chip was prepared based on self-assembly of the thiol-derivatized oligonucleotide, and used for the determination of single-stranded binding protein (SSB) by surface plasmon resonance microscopy (SPR). The experiment results showed that SSB binds ss-DNA with high specificity, and relative signal of SPR response is proportional to the concentration of SSB in the range of 0.1–100 ng/mL with a detection limit (S/N = 3) of 0.07 ng/mL.
Related Topics
Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Jian Quan Lu, Ming Bo Xu, Xing Wang Zhou, Jin Guang Xu, Qing Tao,