Novel preparation of organic polymer-based monolithic column by microwave irradiation

Microwave irradiation was firstly attempted for the preparation of organic-based monoliths of poly(styrene-divinylbenzene-methacrylic acid), which single step in situ polymerization was carried out during 15 min. The column permeability, electrophoretic and chromatographic behaviors were comparatively evaluated using pressure-assisted CEC, CEC and low pressure-driven separation modes. The largest theoretical plates for the preparing column could be close to 18,0000 plates/m for thiourea in the mode of p-CEC. It provided a viable alternative to traditional initiation means for the preparation of monolithic capillary columns.