A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells

In this study, a resorufin derivative RTP-1, which is a novel fluorescent “turn-on” probe for sensitive detection of hydrazine within 30 min, is designed and synthesized. The selective deprotection of the ester group of the probe by hydrazine led to a prominent enhancement of fluorescent intensity, as well as a remarkable color change from colorless to pink, which could be distinguished by naked eye. The fluorescence enhancement showed decent linear relationship with hydrazine concentration ranging from 0 to 50 μmol/L, with a detection limit of 0.84 μmol/L. The specificity of RTP-1 for hydrazine to a number of metal ions, anions and amines is satisfactory. The sensing mechanism of RTP-1 and hydrazine was evaluated by HPLC, ESI mass spectrometry and density functional theory (DFT). Moreover, we have utilized this fluorescent probe for imaging hydrazine in living cells, and the fluorescence was clearly observed when the cells were incubated with hydrazine (100 μmol/L) for 30 min.

Graphical abstractA novel resorufin based fluorescent “turn-on” probe could detect hydrazine levels rapidly and applied in living cells.Figure optionsDownload full-size imageDownload as PowerPoint slide