| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1257214 | Chinese Chemical Letters | 2015 | 5 Pages |
Ionic liquids (ILs) immobilized on silica as novel high performance liquid chromatography (HPLC) stationary phases have attracted considerable attention. However, it has not been applied to protein separation. In this paper, N-methylimidazolium IL-modified silica-based stationary phase (SilprMim) was prepared and investigated as a novel multi-interaction stationary phase charged positively for protein separation. The results indicate that all of the basic proteins tested cannot be absorbed on this novel stationary phase, whereas all of the acidic proteins tested can be retained, and the baseline separation of eight kinds of acidic protein standards can be achieved when performed in reversed phase/ion-exchange chromatography (RPLC/IEC) mode. Compared with commonly used commercial octadecylated silica (ODS) column, the novel stationary phase can show selectivity and good resolution to acidic proteins, which has a promising application in the separation and analyses of acidic proteins from the complex samples in proteomics. In addition, the chromatographic behavior of proteins, the effect of the ligand structure and the retention mechanism on this stationary phase were also investigated.
Graphical abstractA novel silica-based stationary phase modified with N-methylimidazolium ionic liquid was applied to protein separation in RPLC/IEC mode, and showed selectivity and good resolution to acidic proteins, which can lead to a new technology to separate and analyze acidic proteins from the complex samples in proteomics.Figure optionsDownload full-size imageDownload as PowerPoint slide
