Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1258174 | Chinese Chemical Letters | 2009 | 5 Pages |
Abstract
A pH-responsive polymer Eudragit S-100 has been found to assist in correct folding of FGF-2 (fibroblast growth factor-2) denatured with 8Â mol/L urea and 10Â mmol/L dithiothreitol at pH 7.2. The refolding of FGF-2 was performed by directly diluting denatured FGF-2 into a refolding buffer containing Eudragit S-100. The ability of Eudragit S-100 to enhance protein refolding level was investigated using MTT method, fluorescence emission spectroscopy and reverse phase HPLC. On the other hand, the result shows the ability of Eudragit S-100 to enhance the refolding level of protein is due to the interaction between Eudragit S-100 and positively charged FGF-2.
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Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Zhi Feng Huang, Shan Shan Wang, Chun Yan Ni, Shu Lin Yang, Xiao Kun Li, Susanna S.J. Leong,