| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2040222 | Cell Reports | 2014 | 9 Pages |
•A high-resolution method is used to measure DNA-end resection•BRCA1 interaction with CtIP is not essential for resection•BRCA1 interaction with CtIP affects resection speed
SummaryDNA-end resection is a highly regulated and critical step in the response and repair of DNA double-strand breaks. In higher eukaryotes, CtIP regulates resection by integrating cellular signals via its posttranslational modifications and protein-protein interactions, including cell-cycle-controlled interaction with BRCA1. The role of BRCA1 in DNA-end resection is not clear. Here, we develop an assay to study DNA resection in higher eukaryotes at high resolution. We demonstrate that the BRCA1-CtIP interaction, albeit not essential for resection, modulates the speed at which this process takes place.
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