Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2040537 | Cell Reports | 2016 | 12 Pages |
•Co-localization and literature analysis reveals global epigenomic communication network•5hmC is the most-influential hub in the epigenomic communication network of mESCs•Proteins editing and reading 5hmC signals co-evolve•5hmC communicates between different epigenomic processes through specific partners
SummaryEpigenetic communication through histone and cytosine modifications is essential for gene regulation and cell identity. Here, we propose a framework that is based on a chromatin communication model to get insight on the function of epigenetic modifications in ESCs. The epigenetic communication network was inferred from genome-wide location data plus extensive manual annotation. Notably, we found that 5-hydroxymethylcytosine (5hmC) is the most-influential hub of this network, connecting DNA demethylation to nucleosome remodeling complexes and to key transcription factors of pluripotency. Moreover, an evolutionary analysis revealed a central role of 5hmC in the co-evolution of chromatin-related proteins. Further analysis of regions where 5hmC co-localizes with specific interactors shows that each interaction points to chromatin remodeling, stemness, differentiation, or metabolism. Our results highlight the importance of cytosine modifications in the epigenetic communication of ESCs.
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