In Vivo Monosynaptic Excitatory Transmission between Layer 2 Cortical Pyramidal Neurons

•Targeted whole-cell recordings from monosynaptically connected neurons in vivo•Sparse but non-random connectivity between layer 2 pyramidal neurons in vivo•Reduced failure rate and trial-by-trial reliability with increasing uEPSP amplitude•Weak short-term synaptic plasticity

SummaryLittle is known about the properties of monosynaptic connections between identified neurons in vivo. We made multiple (two to four) two-photon targeted whole-cell recordings from neighboring layer 2 mouse somatosensory barrel cortex pyramidal neurons in vivo to investigate excitatory monosynaptic transmission in the hyperpolarized downstate. We report that pyramidal neurons form a sparsely connected (6.7% connectivity) network with an overrepresentation of bidirectional connections. The majority of unitary excitatory postsynaptic potentials were small in amplitude (<0.5 mV), with a small minority >1 mV. The coefficient of variation (CV = 0.74) could largely be explained by the presence of synaptic failures (22%). Both the CV and failure rates were reduced with increasing amplitude. The mean paired-pulse ratio was 1.15 and positively correlated with the CV. Our approach will help bridge the gap between connectivity and function and allow investigations into the impact of brain state on monosynaptic transmission and integration.

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