Kainate Receptors Coexist in a Functional Complex with KCC2 and Regulate Chloride Homeostasis in Hippocampal Neurons

•Native KCC2 exists in a macromolecular complex that contains kainate receptors (KARS)•Deletion of GluK1/2 KARs reduces KCC2 oligomerization and surface expression•Acute silencing and genetic deletion of KARs decreases KCC2-mediated Cl− extrusion•An ionotropic glutamate receptor can positively regulate the function of KCC2

SummaryKCC2 is the neuron-specific K+-Cl− cotransporter required for maintaining low intracellular Cl−, which is essential for fast inhibitory synaptic transmission in the mature CNS. Despite the requirement of KCC2 for inhibitory synaptic transmission, understanding of the cellular mechanisms that regulate KCC2 expression and function is rudimentary. We examined KCC2 in its native protein complex in vivo to identify key KCC2-interacting partners that regulate KCC2 function. Using blue native-polyacrylamide gel electrophoresis (BN-PAGE), we determined that native KCC2 exists in a macromolecular complex with kainate-type glutamate receptors (KARs). We found that KAR subunits are required for KCC2 oligomerization and surface expression. In accordance with this finding, acute and chronic genetic deletion of KARs decreased KCC2 function and weakened synaptic inhibition in hippocampal neurons. Our results reveal KARs as regulators of KCC2, significantly advancing our growing understanding of the tight interplay between excitation and inhibition.

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