Article ID Journal Published Year Pages File Type
2041532 Cell Reports 2016 13 Pages PDF
Abstract

•A genome-wide map of transcription start sites (TSSs) for P. falciparum•Sequence and chromatin features for core promoters of malaria parasites are defined•Dynamic study of TSS usage identifies developmentally regulated transcript isoforms

SummaryA comprehensive map of transcription start sites (TSSs) across the highly AT-rich genome of P. falciparum would aid progress toward deciphering the molecular mechanisms that underlie the timely regulation of gene expression in this malaria parasite. Using high-throughput sequencing technologies, we generated a comprehensive atlas of transcription initiation events at single-nucleotide resolution during the parasite intra-erythrocytic developmental cycle. This detailed analysis of TSS usage enabled us to define architectural features of plasmodial promoters. We demonstrate that TSS selection and strength are constrained by local nucleotide composition. Furthermore, we provide evidence for coordinate and stage-specific TSS usage from distinct sites within the same transcription unit, thereby producing transcript isoforms, a subset of which are developmentally regulated. This work offers a framework for further investigations into the interactions between genomic sequences and regulatory factors governing the complex transcriptional program of this major human pathogen.

Graphical AbstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Related Topics
Life Sciences Agricultural and Biological Sciences Agricultural and Biological Sciences (General)
Authors
, , , , ,