Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase

•Condensin recruitment to centromeric regions requires DNA replication•Centromeric condensin spreads to chromosome arms during anaphase•Condensin promotes recruitment of Top2 during anaphase•Condensin localization requires Polo kinase and correlates with DNA overwinding

SummaryCondensin is a conserved chromosomal complex necessary to promote mitotic chromosome condensation and sister chromatid resolution during anaphase. Here, we report that yeast condensin binds to replicated centromere regions. We show that centromeric condensin relocalizes to chromosome arms as cells undergo anaphase segregation. We find that condensin relocalization is initiated immediately after the bipolar attachment of sister kinetochores to spindles and requires Polo kinase activity. Moreover, condensin localization during anaphase involves a higher binding rate on DNA and temporally overlaps with condensin’s DNA overwinding activity. Finally, we demonstrate that topoisomerase 2 (Top2) is also recruited to chromosome arms during anaphase in a condensin-dependent manner. Our results uncover a functional relation between condensin and Top2 during anaphase to mediate chromosome segregation.

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