Functional Diversification of Maize RNA Polymerase IV and V Subtypes via Alternative Catalytic Subunits

•Alternative second subunits yield two Pol IV and three Pol V subtypes in maize•Pol IV/Pol V subtypes are nonredundant for development and paramutation•Only the largest subunit discriminates Pol IV from Pol V in maize•Pols IV and V associate with common, as well as different, protein partners

SummaryUnlike nuclear multisubunit RNA polymerases I, II, and III, whose subunit compositions are conserved throughout eukaryotes, plant RNA polymerases IV and V are nonessential, Pol II-related enzymes whose subunit compositions are still evolving. Whereas Arabidopsis Pols IV and V differ from Pol II in four or five of their 12 subunits, respectively, and differ from one another in three subunits, proteomic analyses show that maize Pols IV and V differ from Pol II in six subunits but differ from each other only in their largest subunits. Use of alternative catalytic second subunits, which are nonredundant for development and paramutation, yields at least two subtypes of Pol IV and three subtypes of Pol V in maize. Pol IV/Pol V associations with MOP1, RMR1, AGO121, Zm_DRD1/CHR127, SHH2a, and SHH2b extend parallels between paramutation in maize and the RNA-directed DNA methylation pathway in Arabidopsis.

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