Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2060846 | Mycoscience | 2013 | 5 Pages |
Abstract
Agrobacterium tumefaciens-mediated transformation (AMT) was successfully applied to mycelia of the 3 economically important mushrooms Hypsizigus marmoreus, Flammulina velutipes, and Grifola frondosa. We used the hygromycin B resistance gene (hph) under the control of the Cryptococcus neoformans actin promoter. Eighty-six resistant strains of H. marmoreus, 4 of F. velutipes, and 2 of G. frondosa were obtained. All transformants were highly resistant to hygromycin B, suggesting that the C. neoformans actin promoter has a potential universal promoter activity in basidiomycetes. Southern analysis revealed random but single integration of the hph gene.
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Authors
Kanako Hatoh, Kosuke Izumitsu, Atsushi Morita, Kiminori Shimizu, Akira Ohta, Masataka Kawai, Takashi Yamanaka, Hitoshi Neda, Yuko Ota, Chihiro Tanaka,