| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2431846 | Fish & Shellfish Immunology | 2013 | 7 Pages |
IL-17 from pearl oyster Pinctada fucata, one of mollusk, was identified and characterized, and its genomic structure and promoter were analyzed. The full-length cDNA of P. fucata IL-17 (PfIL-17) is 907 bp with an open reading frame of 585 bp encoding a putative protein of 194 amino acids. The deduced PfIL-17 contains a 19 amino acid signal peptide and a conserved IL-17 domain. Multiple sequence alignments and phylogenetic analysis revealed that PfIL-17 has lower similarity with other invertebrate IL-17 and was clustered with CgIL-17, but not clustered with other invertebrate IL-17. Gene expression analysis indicated that PfIL-17 took part in the immune response to LPS and poly(I:C) stimulation, and dual-luciferase reporter assays showed that PfIL-17 could active vertebrate target genes containing the NF-κB binding site and involve NF-κB signal pathway in HEK293 cells. Combined with the results mentioned above, it is suggested that PfIL-17 might involve and activate NF-κB signal pathway against extracellular pathogens.
► IL-17 gene was isolated and characterized from Pinctada fucata. ► PfIL-17 was clustered with CgIL-17, but not clustered with other invertebrate IL-17. ► PfIL-17 involved in the immune response to LPS and poly(I:C) stimulation. ► PfIL-17 could active NF-κB signal pathway in vitro.
