| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2432126 | Fish & Shellfish Immunology | 2011 | 7 Pages |
The cytochemical, immunocytochemical and ultrastructural characteristics of leukocytes and thrombocytes in the peripheral blood of the fat snook (Centropomus paralellus) – a fish occurring in Brazil – were investigated. The cytochemical methods were performed to demonstrate four enzymatic reactions – o-toluidine-hydrogen peroxide, naphtol AS-MX phosphate, naphtol AS-BI phosphate and alpha-naphtil acetate to detect myeloperoxidase (MPO), alkaline phosphatase (ALP), acid phosphatase (ACP) and non-specific esterase (α-NAE), respectively – and two non-enzymatic ones – Periodic-Acid Schiff (PAS) and Sudan black B (SBB) to detect the occurrence of glycogen and phospholipids, respectively. Immunocytochemical method utilizing polyclonal rabbit antibody against mammal metalloproteinases (MMPs) 2 and 9 were done. Standard method for Electron Microscopy (EM) was applied for the ultrastructural study. The cytochemical reactions were positive in neutrophils for MPO, ACP, α-NAE, glycogen and phospholipids; in lymphocytes for ACP and α-NAE; in monocytes for ACP and α-NAE and in thrombocytes for ACP, α-NAE and glycogen. Only neutrophils were positive for MMPs 2 and 9, and none of the cells studied were positive for ALP. Ultrastructurally: 1) neutrophil showed a spherical shape with a spherical, indented or lobulated euchromatic nucleus, and cytoplasm containing granules of varied sizes and mitochondria of varied shapes and sizes. The nucleus/cytoplasm relation and the size of granules suggest neutrophil maturation in peripheral blood; 2) lymphocytes showed partially heterochromatic nucleus and minimal cytoplasm; 3) monocytes had long cytoplasmic projections, an indented nucleus, evident nucleolus and cytoplasm with granules of varied sizes and vacuoles; 4) thrombocytes were predominantly elliptical or roughly spherical in shape, had a partially heterochromatic nucleus and cytoplasm containing electron-dense granules, intricate canalicular system and vacuoles occasionally holding phagocytic material.
► Cytochemistry, immunocytochemistry and ultrastructure of leukocytes and thrombocytes of C. parallelus. ► Reactions were performed to demonstrate MPO, ALP, ACP, α-NAE, PAS, SBB and MMPs 2 and 9. ► The positivity for ACP can detect young and mature neutrophils as well as thrombocytes. ► Nucleus/cytoplasm relation and the size of granules suggest neutrophil maturation in peripheral blood. ► Thrombocytes cytoplasm contain vacuoles occasionally holding phagocytic material.
